Department Facilities and locationsResearch platformsSanger sequencing

Sanger sequencing

Here is the professional English translation for the Applied Biosystems 3500 Dx and Sanger sequencing platform section:

The Applied Biosystems™ 3500 Dx Genetic Analyzer is a capillary electrophoresis sequencing platform based on Sanger technology, designed for research applications that require high accuracy and reliability in DNA fragment reading. The system guarantees reproducible, high-quality results.

The Sanger platform is widely utilized for applications such as genetic variant confirmation, point mutation analysis, genotyping, and targeted molecular biology studies for individual genes and locus-specific regions. The 8-capillary configuration enables an efficient, automated workflow suitable for small- to medium-sized workloads, delivering rapid and standardized turnaround times.

The Sanger sequencing platform includes:

• Applied Biosystems™ 3500 Dx Genetic Analyzer (8-capillary system).
• Dedicated software for sequence acquisition, analysis, and interpretation (Sequencing Analysis Software).
• Specific reagents and consumables for Sanger sequencing (polymers, buffers, capillaries, and reaction kits).
• Standard operating procedures (SOPs) for DNA sequencing and fragment analysis, with potential optimization for specific applications.
• Benchtop equipment and disposable plasticware for sample preparation and loading:
  • Micropipettes (variable ranges) and sterile filter tips.
  • Benchtop microcentrifuges.
  • Vortex mixers.
  • Dry baths (heating blocks) and thermal cyclers (PCR machines).
  • DNA quantification systems (Qubit fluorometer, NanoDrop spectrophotometer).
  • Systems for amplicon quality verification (gel electrophoresis).