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NGS (Next Generation Sequencing)

Here is the professional English translation for the Illumina NextSeq 2000 and NGS platform section:

The Illumina NextSeq 2000 sequencer is a highly flexible and scalable high-throughput sequencing system designed for medium- to high-capacity applications. The service provided enables the generation of high-quality, reliable data with an output of up to 360 Gb per run, adaptable to diverse experimental requirements.

The platform supports a wide range of applications, including exome sequencing, targeted panel analysis, transcriptomics (RNA-seq), single-cell studies, and emerging techniques such as spatialomics. The option to choose from multiple flow cell types, featuring varying levels of capacity and throughput, allows for project-specific customization. This makes the platform suitable for both small-scale studies and complex projects, while optimizing time and cost efficiency.

The NGS sequencing platform includes:

  • NextSeq 2000 Sequencer (compatible with XLEAP-SBS™ chemistry).
  • Web connectivity for run configuration and data storage within the user's BaseSpace cloud environment.
  • Standard operating procedures (SOPs) for primary sequencing applications, as well as the optimization of custom protocols tailored to specific research needs.
  • Hardware and software systems for the acquisition of instrument-generated data and the evaluation of sequencing quality metrics.
  • Common reagents and materials required for sequencing experiments, including reagents for instrument operation, cleaning cycles, and calibration.
  • Benchtop equipment and disposable plasticware for loading libraries into the flow cell:
    • Micropipettes (variable ranges) and sterile filter tips.
    • Benchtop microcentrifuges.
    • Vortex mixers.
    • Dry baths (heating blocks) and thermal cyclers (PCR machines).
    • Quantification systems (Qubit fluorometer, NanoDrop spectrophotometer).
    • Library quality control analysis systems (Bioanalyzer)

 

Last update

04.06.2026

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