Department Facilities and locationsResearch platformsNGS (Next Generation Sequencing)
NGS (Next Generation Sequencing)
Here is the professional English translation for the Illumina NextSeq 2000 and NGS platform section:
The Illumina NextSeq 2000 sequencer is a highly flexible and scalable high-throughput sequencing system designed for medium- to high-capacity applications. The service provided enables the generation of high-quality, reliable data with an output of up to 360 Gb per run, adaptable to diverse experimental requirements.
The platform supports a wide range of applications, including exome sequencing, targeted panel analysis, transcriptomics (RNA-seq), single-cell studies, and emerging techniques such as spatialomics. The option to choose from multiple flow cell types, featuring varying levels of capacity and throughput, allows for project-specific customization. This makes the platform suitable for both small-scale studies and complex projects, while optimizing time and cost efficiency.
The NGS sequencing platform includes:
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NextSeq 2000 Sequencer (compatible with XLEAP-SBS™ chemistry).
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Web connectivity for run configuration and data storage within the user's BaseSpace cloud environment.
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Standard operating procedures (SOPs) for primary sequencing applications, as well as the optimization of custom protocols tailored to specific research needs.
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Hardware and software systems for the acquisition of instrument-generated data and the evaluation of sequencing quality metrics.
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Common reagents and materials required for sequencing experiments, including reagents for instrument operation, cleaning cycles, and calibration.
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Benchtop equipment and disposable plasticware for loading libraries into the flow cell:
- Micropipettes (variable ranges) and sterile filter tips.
- Benchtop microcentrifuges.
- Vortex mixers.
- Dry baths (heating blocks) and thermal cyclers (PCR machines).
- Quantification systems (Qubit fluorometer, NanoDrop spectrophotometer).
- Library quality control analysis systems (Bioanalyzer)
